A Study On Using Enhanced Efficeincy - 1195 Words

In order to improve photolytic deprotection with enhanced efficeincy, several other photoremovable groups have been developed. Figure X.X.X shows o-nitrobenzyl protecting group together with other common photolabile protecting groups such as 1-(o-nitrophenyl)ethyl (NPE), 2-(o-nitrophenyl)-propyl (NPP), 6-nitroveratryloxycarbonyl (NVOC), 2,2-bis(2-nitrophenyl)ethoxycarbonyl (diNPEOC), 4-N-[2-(2-nitropehnyl)-propoxy]carbonyl (NPPOC), 1-(3-nitrodibenzofuran-1-yl)ethyl (NDBF), 6-nitropiperonyloxymethyl (NPOM), p-hydroxyphenacyl (pHP), 7-(diethylaminocoumarin-4-yl)methoxy carbonyl (DEACM), and 6-bromo-7-hydroxycoumarinyl-4-yl-methyl (Bhcmoc) and 6-bromo-7-methoxycoumarinyl-4-yl-methyl (Bmcmoc). A combination of NPOM and NDBF caging group†¦show more content†¦Rodrigues-Correia et al., 2013 and 2014 Ohtsuki et al., 2016 R = H, 6-bromo-7-hydroxycoumarinyl-4-yl-methyl (Bhcmoc) R = Me, 6-bromo-7-methoxycoumarinyl-4-yl-methyl (Bmcmoc) C(N4) A(N6) Furuta et al., 2007 and references therein 1-(3-nitrodibenzofuran-1-yl)ethyl (NDBF) T(N3) UV irradiation at 365 nm Lusic et al., 2010 Figure X.X.X. Photolabile nucleobase protection. Photolabile nucleobase protection has been used to achieve exquisite control over biological processes through light regulation, also referred as photocaging. The concept was introduced by Engels (Engels and Schlaeger, 1977; Engels and Reidys, 1978) and independently by Hofmann (Kaplan et al., 1978). Watson–Crick base pairing is blocked by caging groups, completely preventing oligonucleotide duplex formation. Duplex formation is restored when irradiated by the light at specfic wavelength. Photolabile groups when introduced at specific locations render the molecule inactive; however, upon irradiation with light at particular wavelength, the molecules can be activated (See detailed review by Shao and Xing, 2010; Tang et al. 2013; Liu and Deiters, 2014). Caging groups can also be introduced at phosphate, 2ï‚ ¢-OH, and nucleobases (Tang et al., 2013); however, nucleobase caging has been of particular interest. Oligonucleotides containing NVOC protecting groups have used as the photocleavable protecting group at the N3 position of